Henrique Fonseca Sousa do Nascimento, Plínio Cerqueira dos Santos Cardoso, Helem Ferreira Ribeiro, Tatiane Cristina Mota, Lorena Monteiro Gomes, André Salim Khayat, Adriana Costa Guimarães, Marucia Irena Medeiros Amorim, Rommel Rodriguéz Burbano, Marcelo de Oliveira Bahia
In vitro assessment of anticytotoxic and antigenotoxic effects of CANOVA®
Homeopathy, 2016, 105 (3), 265-269
Background – CANOVA® (CA) is a homeopathic immunomodulator. It contains several homeopathic medicines prepares according to the Brazilian Pharmacopoeia. CA is indicated in clinical conditions in which the immune system is impaired and against tumors. N-methyl-N-nitrosourea (NMU) is an N-nitroso compound, with genotoxic/mutagenic properties. Although several studies have shown promising results in the use of CA, there are no studies reporting possible antigenotoxic effects.
Method – This study evaluated the in vitro antigenotoxic and anticytotoxic effects of CA in human lymphocytes exposed to NMU. Samples of human lymphocytes that were subjected to different concentrations of a mixture containing CA and NMU were used. The genotoxicity/antigenotoxicity of CA was evaluated by the comet assay, anticytotoxicity was assessed by quantification of apoptosis and necrosis using acridine orange/ethidium bromide.
Results – CA significantly reduced DNA damage induced by NMU and reduced significantly the frequency of NMU-induced apoptosis after 24 h of treatment.
Conclusion – CA has an important cytoprotective effect significantly reducing the DNA damage and apoptosis induced by the carcinogen NMU.
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